The role of phytohormones in the induction of morphogenesis by Calycanthus floridus L. explants for in vitro propagation
Keywords:Calycanthus L., decorativeness, nutrient media, multiplication coefficient, growth regulating substances.
AbstractAims. Induction of morphogenesis in vitro by the cultivation of explants on nutrient media depends on many interrelated factors. The main inducers and coordinators of morphogenic processes in vitro are phytohormones, which are introduced into nutrient media in certain concentrations and play a major role in regulating the growth and development of explants. Therefore, elucidation of the morphogenic potential dependence of explants of Clycanthus floridus L. the effect of exogenous growth regulators (phytohormones) is determined by the aim of the research. Materials and methods. The work was conducted in the Plant Micropropagation Laboratory of the National Dendrological Park "Sofiyivka" of the National Academy of Sciences of Ukraine. Plant material C. floridus was used for research obtained from seeds sprouted in vitro. To determine the efficiency of growth, development, and morphogenic potential of C. floridus explants used basal nutrient media according to Murashige and Skoog (MS) and Driver and Kuniyuki Walnut (DKW). Morphogenesis was stimulated by exogenous phytohormones: 6-benzylaminopurine (BAP) – 0.1–2.0 mg/l, β-indolylbutyric acid (IBA) – 0.01–1.00 mg/L and cysteine – 0.5–2.0 mg/l. Results and discussion.The data obtained during the experiment showed that the growth, development and increase of the morphogenic potential of explants, and, accordingly, the multiplication coefficient, largely depend on the content and concentrations of phytohormones in nutrient media. In explants C. floridus cultured on nutrient media according to Murashige and Skoog, modified with the addition of 0.5 mg/L 6-BAP and 0.08 β-IBA and Driver and Kuniyuki with a content of 0.5 mg/L 6-BAP; 0.08 mg/l β-IBA and 1.0 g/L cysteine, active morphogenesis processes and an increase in the multiplication coefficient were detected. Conclusions. The morphogenic capacity of C. floridus explants was investigated. It was found that the active passage of morphogenesis processes in explants and an increase in the multiplication coefficient indicators were facilitated by modification of nutrient media prescribed by Murashige and Skoog with the addition of 0.5 mg/L 6-BAP and 0.08 β-IBA and Driver and Kuniyuki with the content of 0.5 mg/L 6-BAP; 0.08 mg/l β-IBA; 1.0 g/L cysteine.
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