The role of phytohormones in the induction of morphogenesis by Calycanthus floridus L. explants for in vitro propagation

Authors

  • Larysa Koldar
  • Valentyna Oksantiuk

DOI:

https://doi.org/10.37555/2707-3114.16.2020.219819

Keywords:

Calycanthus L., decorativeness, nutrient media, multiplication coefficient, growth regulating substances.

Abstract

Aims. Induction of morphogenesis in vitro by the cultivation of explants on nutrient media depends on many interrelated factors. The main inducers and coordinators of morphogenic processes in vitro are phytohormones, which are introduced into nutrient media in certain concentrations and play a major role in regulating the growth and development of explants. Therefore, elucidation of the morphogenic potential dependence of explants of Clycanthus floridus L. the effect of exogenous growth regulators (phytohormones) is determined by the aim of the research. Materials and methods. The work was conducted in the Plant Micropropagation Laboratory of the National Dendrological Park "Sofiyivka" of the National Academy of Sciences of Ukraine. Plant material C. floridus was used for research obtained from seeds sprouted in vitro. To determine the efficiency of growth, development, and morphogenic potential of C. floridus explants used basal nutrient media according to Murashige and Skoog (MS) and Driver and Kuniyuki Walnut (DKW). Morphogenesis was stimulated by exogenous phytohormones:    6-benzylaminopurine (BAP) – 0.1–2.0 mg/l, β-indolylbutyric acid (IBA) – 0.01–1.00 mg/L and cysteine – 0.5–2.0 mg/l. Results and discussion.The data obtained during the experiment showed that the growth, development and increase of the morphogenic potential of explants, and, accordingly, the multiplication coefficient, largely depend on the content and concentrations of phytohormones in nutrient media. In explants C. floridus cultured on nutrient media according to Murashige and Skoog, modified with the addition of 0.5 mg/L 6-BAP and 0.08 β-IBA and Driver and Kuniyuki with a content of 0.5 mg/L 6-BAP; 0.08 mg/l β-IBA and 1.0 g/L cysteine, active morphogenesis processes and an increase in the multiplication coefficient were detected. Conclusions. The morphogenic capacity of C. floridus explants was investigated. It was found that the active passage of morphogenesis processes in explants and an increase in the multiplication coefficient indicators were facilitated by modification of nutrient media prescribed by Murashige and Skoog with the addition of 0.5 mg/L 6-BAP and 0.08 β-IBA and Driver and Kuniyuki with the content of 0.5 mg/L 6-BAP; 0.08 mg/l β-IBA; 1.0 g/L cysteine.

References

Calycanthus L. (2020). World Checklist of Selected Plant Families. URL: http://apps.kew. org/wcsp/.

Diels, L. (1912). Über primitive Ranales der australischen Flora. Botanische Jahrbücher fur Systematik, Pflanzengeschichte und Pflanzengeographie. 48, Beiblatt zu den Botanischen Jahrbüchern. Nr. 107. S. 7–13. (in German).

Driver, J., & Kuniyuki, A. (1984). In vitro propagation of Paradox walnut rootstock. Horticultural Science. Vol. 19. P. 507–509.

Govaerts, R. (1999). World Checklist of Seed Plants 3(1, 2a & 2b). Deurne: MIM. 1–1532.

Grant, V. (1950). The pollination of Calycanthus occidentalis. American Journal of Botany. Vol. 37(4). P. 294–297.

Kalynyn, F. L., Sarnatskaia, V. V., & Polyshchuk, V. E. (1980). Metody kul'tury tkaney v fyzyolohyy y byokhymyy rastenyy. Kyiv: Naukova dumka, 488 s. (in Russian).

Kataeva, N. V., & Butenko, R. H. (1983). Klonal'noe mykrorazmnozhenye rastenyy. Moskva: Nauka, 96 s. (in Russian).

Koldar, L. A. (2015). Perspektyvy vykorystannia vydiv rodu Calycanthus Lindl. u zelenomu budivnytstvi Pravoberezhnoho Lisostepu Ukrainy. Materialy mizhnar. nauk. konf. «Aktual'ni problemy sadovo-parkovoho mystetstva» 27–28 travnia 2015 r. Uman'. 2015. S. 93–95. (in Ukrainian).

Kruglova, N. N., & Seldimirova, O. A. (2013). The pathways of morphogenesis in vitro of wheat androclynic callus cells. Plant physiology and genetics. Vol. 45. No 5. P. 382–389. (in Russian).

Melnychuk M. D., Novak T. V., & Kunakh V. A. (2003). Biotekhnolohiia roslyn. Kyiv: Vyshcha osvita. 520 s. (in Ukrainian).

Moerman, D. E. (1998). Native American Etnobotany. Portland, OR: Timber Press, 927 p.

Murashige, T. М., & Skoog, F. K. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 1962. Vol. 15. Р. 473–497.

Oleshko, V. V., & Havryliuk, O. S. (2011). Introduktsiia vydiv rodu Kalikantus (Calycanthus L.) ta perspektyvy vprovadzhennia ikh v ozelenennia. Naukovyy visnyk VNU im. Lesi Ukrainky. No 19. S. 30–31. (in Ukrainian).

Oleshko, V. V., Havrylyuk O. S. (2012). Vegetative propagation of species of strawberry shrub Calycanthus L. genus in the Volyn Forest-steppe of Ukraine. News Biosphere Reserve «Askania-Nova». No 14. P. 190–194. (in Ukrainian).

Takhtajan, A. L. (2009), Flowering plants [corr. 2nd ed.]. N. Y. Springer Science+Business Media, 871 p.

Zhang, R., & Liu, H. (1998). Wax shrubs in world:('Calycanthaceae'). China Science and Technology Press. P. 1–15.

Zhou, S., Renner, S. S., & Wen, J. (2006). Molecular phylogeny and intra-and intercontinen-tal biogeography of Calycanthaceae. Molecular Phylogenetics and Evolution, Vol. 39(1), P. 1–15.

Zhuravlev, Yu. N., & Omel'ko, A. M. (2008). Morfohenez u rastenyy in vitro. Fyzyolohyia rastenyy. Moskva: T. 55. No 5. S. 643–664. (in Russian).

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