Karyotype and plasmotype characteristics in wheat-rye hybrids with the different structural organization of the nuclear genome

Authors

  • E. A. Sycheva
  • A. V. Sokoluk
  • M. E. Vasilevskaya
  • L. A. Solovey
  • E. B. Bondarevich
  • O. M. Lyusikov
  • N. I. Dubovets

DOI:

https://doi.org/10.37555/2707-3114.1.2021.247730

Keywords:

пшенично-житні гібриди, хромосомно-заміщені лінії, каріотип, плазмотіп, С-бендінг, ДНК-маркери

Abstract

Aim. Genome structure analysis and plasmotype identification in wheat-rye hybrids of various types (triti- cale, secalotriticum) and ploidy level. Mcth0ds. Cytological and molecular-genetic analysis. Rcsults. The karyotype and plasmotype analysis was carried out in 11 stable lines of secondary recombinant hexaploid triticale with the introgression of D-genome chromosomes of the wheat (A/B/DRR, 2n = 6x = 42), 14 stable and highly productive secalotriticum lines of F6–16 generations (Secalotriticum, S/RRAABB, 2n = 6x = 42), 9 stable lines of tetraploid triticale (A/BRR, 2n = 4x = 28). By means of differential chromosome staining, the chromosomal composition of the experimental material was characterized and the intergenomic substitution and translocation of chromosomes were detected. The PCR-RFLP analysis of the 18S/5S mitochondrial (mt) repeat and the ndhH-region of chloroplast DNA showed that these organ- elle DNA regions are in the homoplasmic state and belong to rye-type cytoplasm in secalotriticum lines and wheat-type cytoplasm in tetraploid and secondary recombinant hexaploid triticale lines. C0nclusi0ns. Cytological and molecular genetic analysis revealed significant genetic diversity of the created gene pool of wheat-rye hybrids by nuclear-cytoplasmic structure. The synthesized linear material of wheat-rye hybrids may be used in cytogenetic research and practical breeding.

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Published

2021-12-28

How to Cite

Sycheva, E. A., Sokoluk, A. V., Vasilevskaya, M. E., Solovey, L. A., Bondarevich, E. B., Lyusikov, O. M., & Dubovets, N. I. (2021). Karyotype and plasmotype characteristics in wheat-rye hybrids with the different structural organization of the nuclear genome. Journal of Native and Alien Plant Studies, (1), 278–284. https://doi.org/10.37555/2707-3114.1.2021.247730

Issue

No. 1 (2021): Матеріали міжнар. наук. конф. «Охорона біорізноманіття та історико-культурної спадщини у ботанічних садах та дендропарках»

Section

Статті

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