Optimization of paulownia cultivation technology (Paulownia Siebold & Zucc.) in vitro
Keywords:apical bud, micropropagation, macrosalts, kinetin, 6-benzylaminopurine, temperature, artificial lighting.
Aim. Analysis of information on the technology of Paulownia (Paulownia Siebold & Zucc.) in vitro cultivation and its generalization with the results of own research. Methods. The research was carried out under the standard conditions of the in vitro laboratory of the "BERRY FARM UKRAINE" Company. Explants were cultured on nutrient media according to Quarin and Lepouvre (QL) and our recipe (MK). Serial studies were conducted according to the principle: the best version of the previous experiment was a control for the next one. Statistical analysis was performed using Microsoft Excel 1997–2010. Results and Discussion. The results of experimental studies of 2018–2019 on the optimization of the technology of growing paulownia by biotechnological methods were analyzed. As a result, the following have been studied: the influence of various numbers of aseptic cultivation on shoot growth; the reaction of regenerants to changes in the nutrient medium by macrosalts; the influence of temperature, and sucrose concentration on the growth of regenerates; dependence between exposure, light spectrum, and growth of regenerates; synergistic effect of cytokinins and red-blue lighting on the growth of regenerates and formation of vitrified plants; phytotoxicity of cytokinins depending on the acidity of the nutrient medium; their concentration and possibility of combination; the effect of cytokinins concentrations in the nutrient medium and the compensatory effect of mother plant lighting options on the length of the root system of offspring from stem cuttings and other effects. Conclusions. The apical meristem of a bud; combining MK (2/3) and QL (1/3) environments with a pH of 5.6–5.8; with a sucrose content of 3–5%; by adding 0.8 mg/l kinetin + 0.2 mg/l 6-BAP; the optimal temperature is 26 °C; a photoperiod of 16 hours with a combination of monochromatic LEDs of red and blue spectra "4+2" for paulownia micropropagations were efficient.
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